Category: 科學筆記

[Flow cytometry] T cell phenotypes(T cells subsets)gating strategy

To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. By these, you can first gate CD4 and CD8 T cells and then their subsets, such as T Central Memory cells (TCM cells), T Effector Memory cells (TEM cells), T Terminal Effector cells (TTE cells), T stem cell memory, T regulatory cells (Treg cells) and Treg subsets (TregCM cells, TregEM cells and TregTE cells).