{"id":6602,"date":"2020-11-14T09:35:00","date_gmt":"2020-11-14T08:35:00","guid":{"rendered":"https:\/\/myoceane.fr\/?p=6602"},"modified":"2020-11-27T08:20:04","modified_gmt":"2020-11-27T07:20:04","slug":"fastq-%e6%aa%94%e6%a1%88%e4%bb%8b%e7%b4%b9","status":"publish","type":"post","link":"https:\/\/myoceane.fr\/index.php\/fastq-%e6%aa%94%e6%a1%88%e4%bb%8b%e7%b4%b9\/","title":{"rendered":"FASTQ \u6a94\u6848\u4ecb\u7d39"},"content":{"rendered":"<div id=\"fb-root\"><\/div>\n<p style=\"text-align: justify;\">\u96a8\u8457\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u8d8a\u4f86\u8d8a\u666e\u53ca\uff0c\u7522\u751f\u7684\u8cc7\u6599\u8d8a\u4f86\u8d8a\u591a\uff0cFastq \u9019\u7a2e\u63cf\u8ff0\u9e7c\u57fa\u5e8f\u5217 (Nucleotide Sequence) \u7684\u6a94\u6848\u683c\u5f0f\u4e5f\u4f7f\u7528\u7684\u8d8a\u4f86\u8d8a\u591a\uff0c\u672c\u7bc7\u60f3\u8981\u6574\u7406\u4e00\u4e9b\u6709\u95dc Fastq \u6a94\u6848\u4ecb\u7d39\uff0c\u50cf\u662f Fastq Data Format \u8207\u5982\u4f55\u5229\u7528 Fastq \u505a Quality Control (QC)?<\/p>\n<h5>FASTQ Format\uff1a<\/h5>\n<p>\u7db2\u8def\u4e0a\u883b\u591a\u95dc\u65bc FastQ \u683c\u5f0f\u4ecb\u7d39\u7684\u8cc7\u6599\uff0c\u6211\u5011\u5df2\u4ee5\u4e0b\u7684\u7bc4\u4f8b\u4f86\u4ecb\u7d39\u4e00\u500b Fastq \u7684\u6a94\u6848\u901a\u5e38\u6703\u6709\u54ea\u4e00\u4e9b\u8cc7\u8a0a\uff0c\u4ee5\u4e0b\u7684\u8cc7\u8a0a\u662f\u4e00\u500b\u9e7c\u57fa\u5e8f\u5217\u7684\u8868\u793a\u65b9\u6cd5\uff1a<\/p>\n<pre class=\"lang:python gutter:false\">@NB552005:36:HKNVTAFXY:1:11101:2719:1049 2:N:0:ATCACGAC+AAGGTTCA\nCACTTGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAAACCCGTCTCTACTAAAATACAAAAATTAGTCAGGTGATGTGGTGGGCATCTGTAATCCCAGCTACTCGGAAGGCTGAGACAGAAGAATCGCTTGAACCTGGGAAGTGG\n+\nAAAAAEEEEEE\/EEEEE\/EEAAAEAAEEEEA\/EEEEEEEEA\/AEEAEEEAEAEEAEEA&lt;EEEEEEE&lt;A&lt;AEEAA&lt;EEEE\/\/E\/EEEEEEEEEEEAEEEEAEE&lt;EE\/&lt;EAEEEA\/EEEEE\/A\/AEE\/A\/\/&lt;AAEAEAE\/\/AEE&lt;&lt;EEEEAE&lt;<\/pre>\n<p>\u901a\u5e38 Fastq \u88e1\u9762\u6703\u6709\u5f88\u591a\u50cf\u662f\u4ee5\u4e0a\u7684\u9e7c\u57fa\u5e8f\u5217\uff0c\u4e00\u500b\u9e7c\u57fa\u5e8f\u5217\u6703\u7531\u56db\u500b\u90e8\u5206\u7d44\u6210\uff1a<\/p>\n<ol>\n<li>@ \u4e4b\u5f8c\u5305\u542b\u5f88\u591a\u7522\u751f\u9e7c\u57fa\u5e8f\u5217\u7684\u8cc7\u8a0a\uff1a<\/li>\n<li>\u8b80\u53d6\u5230\u7684\u9e7c\u57fa\u5e8f\u5217\uff08\u5305\u542b Adaptor\uff09<\/li>\n<li>+ \u662f\u5206\u9694\u7b26\u865f<\/li>\n<li>\u95dc\u65bc\u9e7c\u57fa\u5e8f\u5217\u7684\u4fe1\u5fc3\u4fe1\u865f<\/li>\n<\/ol>\n<h5>Single-Read Run v.s Paired-End Run <a href=\"https:\/\/emea.support.illumina.com\/bulletins\/2016\/04\/fastq-files-explained.html\">\u53c3\u8003<\/a><\/h5>\n<p style=\"text-align: justify;\">Fastq \u6a94\u6848\u4e3b\u8981\u53ef\u4ee5\u5206\u6210\u5169\u7a2e\uff0c\u4e00\u7a2e\u662f Single-Read\uff0c\u9019\u7a2e Fastq \u7522\u751f\u4e3b\u8981\u662f\u5f9e DNA \u7684\u5176\u4e2d\u4e00\u7aef\u8b80\u9032\u8cc7\u6599\uff0c\u53e6\u5916\u4e00\u7a2e\u662f Paired-End Fastq\uff0c\u9019\u985e Fastq \u4e00\u822c\u6703\u7528\u5169\u500b\u6a94\u6848 (R1, R2) \u63cf\u8ff0\u540c\u4e00\u500b Sample Nucleotide Sequences\uff0cFastq \u7684\u6a94\u6848\u90fd\u662f\u4ee5 *.fastq.gz \u7232\u4ed6\u7684\u6a94\u540d\uff0c\u4ee5\u4e0b\u6211\u5011\u63d0\u4f9b\u4e00\u500b Paired-End \u7684\u4f8b\u5b50\uff1a<\/p>\n<p>Read 1\uff1a(\u5982\u679c\u53ea\u6709\u4e00\u500b\u6a94\u6848 Read 1 \u5c31\u662f Single-Read)<\/p>\n<pre class=\"lang:bash\">@NB552005:36:HKNVTAFXY:1:11101:14761:1033 1:N:0:ATCACGAC+AAGGTTCA\nCCCAGNTTAGAGTGCGATGACGCCATCTCGGCTCACTACAACCTCCATCTCCTGGGTTCAAGTGGTTCTCCTGCCTCAGCCTCCCGAGTAGCAGGGATTACAGGGGCACATTACCACGACCGGCTAATTTCTGTATTTTTAGTAGAGA\n+\nAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE6EEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEEEEEEEEEEEAEEEEEEEEE&lt;EEEE\/EEEEE\/EEEEEEEEE&lt;EEEEAEEEEEEEEEEEEEE<\/pre>\n<p>Read 2\uff1a(\u5982\u679c\u662f Paired-End Fastq \u5c31\u6703\u540c\u6642\u6709 Read 1 \u8207 Read 2)<\/p>\n<pre class=\"lang:bash\">@NB552005:36:HKNVTAFXY:1:11101:14761:1033 2:N:0:ATCACGAC+AAGGTTCA\nTCTCTACTAAAAATACAGAAATTAGCCGGTCGTGGTAATGTGCCCCTGTAATCCCTGCTACTCGGGAGGCTGAGGCAGGAGAACCACTTGAACCCAGGAGATGGAGGTTGTAGTGAGCCGAGATGGCGTCATCGCACTCTAACCTGGG\n+\nAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAEEEEEEEEEE&lt;EEEEEEEEEEEEE&lt;EEEEAEEEEEEEEEEEEEEAEEAAEE\/EEAEEAA\/EEEAAEEE&lt;E\/EEEEEE<\/pre>\n<p>\u7531\u65bc Paired-End Fastq \u662f\u540c\u4e00\u500b DNA \u5f9e\u4e0d\u540c\u7aef\u8b80\u51fa\u4f86\u7684\u7d50\u679c\uff0c\u4f46\u662f\u4e00\u822c\u5b9a\u5e8f\u5100\u7684\u7cbe\u6e96\u5ea6\u5728 150bp \u4e4b\u5f8c\u6703\u5927\u5e45\u4e0b\u964d\uff0cRead1 \u8b80\u5230\u7684\u662f\u5de6\u534a\u90e8\uff0cRead2 \u8b80\u5230\u7684\u662f\u53f3\u534a\u90e8\uff0c\u7136\u5f8c\u4e2d\u9593\u6709\u91cd\u758a\u7684\u90e8\u5206\uff0c\u4ee5\u4e0a\u7684 Sample \u6bd4\u8f03\u7279\u5225\uff0c\u76f4\u63a5 Reverse Read 2 \u5c31\u53ef\u4ee5\u8ddf Read 1 \u5339\u914d\u4e86\uff0c\u9019\u4ee3\u8868\u53d6\u6a23\u7684\u57fa\u56e0\u5e8f\u5217\u5dee\u4e0d\u591a\u5c31\u662f 150 bp \u7684\u9577\u5ea6<\/p>\n<pre class=\"lang:bash\">CCCAGNTTAGAGTGCGATGACGCCATCTCGGCTCACTACAACCTCCATCTCCTGGGTTCAAGTGGTTCTCCTGCCTCAGCCTCCCGAGTAGCAGGGATTACAGGGGCACATTACCACGACCGGCTAATTTCTGTATTTTTAGTAGAGA\nGGGTCCAATCTCACGCTACTGCGGTAGAGCCGAGTGATGTTGGAGGTAGAGGACCCAAGTTCACCAAGAGGACGGAGTCGGAGGGCTCATCGTCCCTAATGTCCCCGTGTAATGGTGCTGGCCGATTAAAGACATAAAAATCATCTCT<\/pre>\n<p>\u518d\u4f86\u770b\u53e6\u5916\u4e00\u500b\u7bc4\u4f8b\uff1a<\/p>\n<p>Read 1\uff1a<\/p>\n<pre class=\"lang:bash\">@NB552005:36:HKNVTAFXY:1:11101:10343:1028 1:N:0:ATCACGAC+AAGGTTCA\nTAATTNGTTTGTTTGTTTGAGATGGAGTTTCGCTCTGTCACCCAGGCTGGAGTATAGTGGCGTGATCTCGGCTCACTGCAACCTCCATCTCCCAGATTCAAGTGAGTCTCCTGCCTCAGCCTGCTGGGTAGTTCAGATTATAGGCATGTGC\n+\nAAAAAEEEEEEEEEEEE&lt;EEEEEEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE\/EEEEEEEEE\/EAEEAEEEEEEEEEEEE&lt;EEEEEEEEE\/EEEEEEEEAEEAEE\/EAEEEEEEAEEEEEEAAE&lt;EEEEEEEEEE\/AAA<\/pre>\n<p>Read 2\uff1a<\/p>\n<pre class=\"lang:bash\">@NB552005:36:HKNVTAFXY:1:11101:10343:1028 2:N:0:ATCACGAC+AAGGTTCA\nCACTTGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGTGAAACCCTGTCTGTACTAAAAATACAAAAATTAGCCAGGCGTGGTGGCACATGCCTATAATCTGAACTACCCAGCAGGCTGAGGCAGGAGACTCACTTGAATCTGGGAGATG\n+\nAAAAAAE\/EEEEEEEEEAEEAEEEEE\/EE&lt;EEEEEE\/AEEEAEEAEEAAEEEAEEEEEAAEEEEEEAE\/EAAA\/EAEEEEEEEEAEA\/EAEEE&lt;EEEEEEEEEAEEAEEEEEEE&lt;EEEEEAEEAAEEEEEAA&lt;\/A&lt;&lt;EE&lt;EEEEEAEEEEE<\/pre>\n<p style=\"text-align: justify;\">\u53ef\u4ee5\u767c\u73fe\u9019\u4e00\u7d44 Read1, Read2 \u5f9e\u7b2c 86 bp \u958b\u59cb\u53ef\u4ee5\u5339\u914d\uff0c\u9019\u4ee3\u8868\u4e86\u9019\u4e00\u500b\u57fa\u56e0\u5e8f\u5217\u9577\u5ea6\u662f 86*2 + (150 &#8211; 86) = 236 bp\uff0c\u5176\u4ed6\u7684 Single-Read \u8ddf Paired-End \u5dee\u5225\u53ef\u4ee5<a href=\"https:\/\/yourgene.pixnet.net\/blog\/post\/68952443-paired-end-v.s.-single-end\">\u53c3\u8003\u7db2\u7ad9<\/a>\u3002<\/p>\n<pre class=\"lang:bash\">CATCTCCCAGATTCAAGTGAGTCTCCTGCCTCAGCCTGCTGGGTAGTTCAGATTATAGGCATGTGC\nGTAGAGGGTCTAAGTTCACTCAGAGGACGGAGTCGGACGACCCATCAAGTCTAATATCCGTACACG<\/pre>\n<h5>How to do Fastq Quality Control?<\/h5>\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"\u9ad8\u901a\u91cf\u5b9a\u5e8f\u7db2\u8def\u7814\u8a0e\u6703  \u9ad8\u901a\u91cf\u5b9a\u5e8f\u6578\u64da\u8655\u7406\u8207\u54c1\u8cea\u63a7\u7ba1\uff0dNGS data QC\" width=\"910\" height=\"512\" src=\"https:\/\/www.youtube.com\/embed\/k0yLCdl09GM?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><figcaption>\u5982\u4f55\u505a FASTQ \u7684 QC (Quality Control) ?<\/figcaption><\/figure>\n\n\n<p>\u5176\u4ed6\u53c3\u8003\u6587\u7ae0\uff1a<a href=\"https:\/\/myoceane.fr\/index.php\/vcf-variant-calling-format\/\">VCF \u2013 Variant Calling Format \u57fa\u56e0\u8b8a\u7570\u5132\u5b58\u683c\u5f0f<\/a><\/p>\n\n\n<figure class=\"wp-block-embed-youtube wp-block-embed is-type-video is-provider-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"StatQuest: A gentle introduction to RNA-seq\" width=\"910\" height=\"512\" src=\"https:\/\/www.youtube.com\/embed\/tlf6wYJrwKY?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u96a8\u8457\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u8d8a\u4f86\u8d8a\u666e\u53ca\uff0c\u7522\u751f\u7684\u8cc7\u6599\u8d8a\u4f86\u8d8a\u591a\uff0cFastq \u9019\u7a2e\u63cf\u8ff0\u9e7c\u57fa\u5e8f\u5217 (Nucleotide Sequence) \u7684\u6a94\u6848\u683c\u5f0f\u4e5f\u4f7f\u7528\u7684\u8d8a\u4f86\u8d8a\u591a\uff0c\u672c\u7bc7\u60f3\u8981\u6574\u7406\u4e00\u4e9b\u6709\u95dc Fastq \u6a94\u6848\u4ecb\u7d39\uff0c\u50cf\u662f Fastq Data Format \u8207\u5982\u4f55\u5229\u7528 Fastq \u505a Quality Control (QC)?<\/p>\n","protected":false},"author":1,"featured_media":6647,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[337,16],"tags":[1000,1001,1002],"class_list":["post-6602","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-bioinformatics","category-health","tag-fastq","tag-fastq-format","tag-quality-control"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v24.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>FASTQ \u6a94\u6848\u4ecb\u7d39 - \u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane<\/title>\n<meta name=\"description\" content=\"\u96a8\u8457\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u8d8a\u4f86\u8d8a\u666e\u53ca\uff0c\u7522\u751f\u7684\u8cc7\u6599\u8d8a\u4f86\u8d8a\u591a\uff0cFastq \u9019\u7a2e\u63cf\u8ff0\u9e7c\u57fa\u5e8f\u5217 (Nucleotide Sequence) \u7684\u6a94\u6848\u683c\u5f0f\u4e5f\u4f7f\u7528\u7684\u8d8a\u4f86\u8d8a\u591a\uff0c\u672c\u7bc7\u60f3\u8981\u6574\u7406\u4e00\u4e9b\u6709\u95dc Fastq \u6a94\u6848\u4ecb\u7d39\uff0c\u50cf\u662f Fastq Data Format \u8207\u5982\u4f55\u5229\u7528 Fastq \u505a Quality Control (QC)?\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/myoceane.fr\/index.php\/fastq-\u6a94\u6848\u4ecb\u7d39\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"FASTQ \u6a94\u6848\u4ecb\u7d39 - \u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane\" \/>\n<meta property=\"og:description\" content=\"\u96a8\u8457\u6b21\u4e16\u4ee3\u5b9a\u5e8f (NGS) \u8d8a\u4f86\u8d8a\u666e\u53ca\uff0c\u7522\u751f\u7684\u8cc7\u6599\u8d8a\u4f86\u8d8a\u591a\uff0cFastq \u9019\u7a2e\u63cf\u8ff0\u9e7c\u57fa\u5e8f\u5217 (Nucleotide Sequence) \u7684\u6a94\u6848\u683c\u5f0f\u4e5f\u4f7f\u7528\u7684\u8d8a\u4f86\u8d8a\u591a\uff0c\u672c\u7bc7\u60f3\u8981\u6574\u7406\u4e00\u4e9b\u6709\u95dc Fastq \u6a94\u6848\u4ecb\u7d39\uff0c\u50cf\u662f Fastq Data Format \u8207\u5982\u4f55\u5229\u7528 Fastq \u505a Quality Control (QC)?\" \/>\n<meta property=\"og:url\" content=\"https:\/\/myoceane.fr\/index.php\/fastq-\u6a94\u6848\u4ecb\u7d39\/\" \/>\n<meta property=\"og:site_name\" content=\"\u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane\" \/>\n<meta property=\"article:published_time\" content=\"2020-11-14T08:35:00+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-11-27T07:20:04+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/myoceane.fr\/wp-content\/uploads\/2020\/11\/fastq.png\" \/>\n\t<meta property=\"og:image:width\" content=\"2132\" \/>\n\t<meta property=\"og:image:height\" content=\"562\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"\u6ab8\u6aac\u7238\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"\u6ab8\u6aac\u7238\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" 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