{"id":4301,"date":"2020-05-09T14:37:16","date_gmt":"2020-05-09T12:37:16","guid":{"rendered":"https:\/\/myoceane.fr\/?p=4301"},"modified":"2020-05-13T10:12:55","modified_gmt":"2020-05-13T08:12:55","slug":"%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89","status":"publish","type":"post","link":"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/","title":{"rendered":"[Flow cytometry] T cell phenotypes\uff08T cells subsets\uff09gating strategy"},"content":{"rendered":"
<\/div>\n\n

How to design a panel for analyzing T cell subsets in peripheral blood by using Flow Cytometry?<\/p>\n\n\n

First of all, you have to know which lasers you have in your machine. Base on the lasers you have, design a panel with suitable fluorochromes conjugated with your cell markers.<\/p>\n

To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. By these, you can first gate CD4 and CD8 T cells and then their subsets, such as T Central Memory cells (\uff34CM<\/sub> cells), T Effector Memory cells (\uff34EM<\/sub> cells), T Terminal Effector cells (\uff34TE<\/sub> cells), T stem cell memory, T regulatory cells (Treg cells) and Treg subsets (\uff34regCM<\/sub> cells, \uff34regEM<\/sub> cells and \uff34regTE<\/sub> cells).<\/p>\n

CD Markers panel\uff08BD biosciences\uff09<\/h5>\n\n\n
  1. CD45<\/strong> KrO<\/li>
  2. CD3<\/strong> APC750<\/li>
  3. CD4<\/strong> APC<\/li>
  4. CD8<\/strong> APC700<\/li>
  5. CD45RA<\/strong> FITC<\/li>
  6. CCR7<\/strong> PE<\/li>
  7. PD-1<\/strong> PC5.5<\/li>
  8. CD57<\/strong> PB<\/li>
  9. CD27<\/strong> PC7<\/li>
  10. CD28<\/strong> ECD<\/li>
  11. CD127<\/strong> BV650<\/li>
  12. CD25<\/strong> BV785<\/li>
  13. CD14<\/strong> BUV395<\/li>
  14. CD16<\/strong> BUV496<\/li>
  15. HLA-DR<\/strong> BUV661<\/li>
  16. CD38<\/strong> BUV525<\/li>
  17. CD95<\/strong> BUV405<\/li>
  18. Promokine 840 viability<\/li>
  19. Forward Scatter<\/li>
  20. Side Scatter<\/li>
  21. Time<\/li><\/ol>\n\n\n
    Here is an example of T cell subsets gating strategy:<\/h5>\n\n\n
    \"\"
    Figure 1: T cell subsets gating strategy<\/strong><\/figcaption><\/figure>\n\n\n\n

    <\/p>\n","protected":false},"excerpt":{"rendered":"

    To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. By these, you can first gate CD4 and CD8 T cells and then their subsets, such as T Central Memory cells (\uff34CM cells), T Effector Memory cells (\uff34EM cells), T Terminal Effector cells (\uff34TE cells), T stem cell memory, T regulatory cells (Treg cells) and Treg subsets (\uff34regCM cells, \uff34regEM cells and \uff34regTE cells).<\/p>\n","protected":false},"author":3,"featured_media":4360,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16,218],"tags":[289,290,291,285,286,312,287,292,294,299,298,296,293,295,300,301,297,288],"class_list":["post-4301","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-health","category-218","tag-cd-marker","tag-cd4","tag-cd8","tag-flow-cytometry","tag-t-cell-phenotypes","tag-t-cell-subsets-gating-strategy","tag-t-cells-subsets","tag-t-central-memory-cells","tag-t-effector-memory-cells","tag-t-regulatory-cells","tag-t-stem-cell-memory","tag-t-terminal-effector-cells","tag-cm-cells","tag-em-cells","tag-treg-cells","tag-treg-subsets","tag-te-cells","tag-288"],"yoast_head":"\n[Flow cytometry] T cell phenotypes\uff08T cells subsets\uff09gating strategy - \u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane<\/title>\n<meta name=\"description\" content=\"To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. By these, you can first gate CD4 and CD8 T cells and then their subsets, such as T Central Memory cells (\uff34CM cells), T Effector Memory cells (\uff34EM cells), T Terminal Effector cells (\uff34TE cells), T stem cell memory, T regulatory cells (Treg cells) and Treg subsets (\uff34regCM cells, \uff34regEM cells and \uff34regTE cells).\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/myoceane.fr\/index.php\/\u5be6\u9a57-flow-cytometry-t-cell-phenotypes\uff08t-cells-subsets\uff09\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"[Flow cytometry] T cell phenotypes\uff08T cells subsets\uff09gating strategy - \u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane\" \/>\n<meta property=\"og:description\" content=\"To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. By these, you can first gate CD4 and CD8 T cells and then their subsets, such as T Central Memory cells (\uff34CM cells), T Effector Memory cells (\uff34EM cells), T Terminal Effector cells (\uff34TE cells), T stem cell memory, T regulatory cells (Treg cells) and Treg subsets (\uff34regCM cells, \uff34regEM cells and \uff34regTE cells).\" \/>\n<meta property=\"og:url\" content=\"https:\/\/myoceane.fr\/index.php\/\u5be6\u9a57-flow-cytometry-t-cell-phenotypes\uff08t-cells-subsets\uff09\/\" \/>\n<meta property=\"og:site_name\" content=\"\u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane\" \/>\n<meta property=\"article:published_time\" content=\"2020-05-09T12:37:16+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-13T08:12:55+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/myoceane.fr\/wp-content\/uploads\/2020\/05\/T-cell-phenotypes-.png\" \/>\n\t<meta property=\"og:image:width\" content=\"2500\" \/>\n\t<meta property=\"og:image:height\" content=\"2437\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"\u6ab8\u6aac\u5abd\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"\u6ab8\u6aac\u5abd\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/#article\",\"isPartOf\":{\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/\"},\"author\":{\"name\":\"\u6ab8\u6aac\u5abd\",\"@id\":\"https:\/\/myoceane.fr\/#\/schema\/person\/4131a9a9e8a1517447647089109fe4f3\"},\"headline\":\"[Flow cytometry] T cell phenotypes\uff08T cells subsets\uff09gating strategy\",\"datePublished\":\"2020-05-09T12:37:16+00:00\",\"dateModified\":\"2020-05-13T08:12:55+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/\"},\"wordCount\":198,\"commentCount\":0,\"publisher\":{\"@id\":\"https:\/\/myoceane.fr\/#\/schema\/person\/4a4552fb8c27693083d465e12db7658b\"},\"image\":{\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/myoceane.fr\/wp-content\/uploads\/2020\/05\/T-cell-phenotypes-.png\",\"keywords\":[\"CD marker\",\"CD4\",\"CD8\",\"Flow cytometry\",\"T cell phenotypes\",\"T cell subsets gating strategy\",\"T cells subsets\",\"T Central Memory cells\",\"T Effector Memory cells\",\"T regulatory cells\",\"T stem cell memory\",\"T Terminal Effector cells\",\"\uff34CM cells\",\"\uff34EM cells\",\"Treg cells\",\"Treg subsets\",\"\uff34TE cells\",\"\u6d41\u5f0f\u7d30\u80de\u5100\"],\"articleSection\":[\"Health \u5065\u5eb7\u4fdd\u5065\",\"\u79d1\u5b78\u7b46\u8a18\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/\",\"url\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/\",\"name\":\"[Flow cytometry] T cell phenotypes\uff08T cells subsets\uff09gating strategy - \u60f3\u65b9\u6d89\u6cd5 - \u91cf\u74f6\u5916\u7684\u5929\u7a7a M-Y-Oceane\",\"isPartOf\":{\"@id\":\"https:\/\/myoceane.fr\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/#primaryimage\"},\"image\":{\"@id\":\"https:\/\/myoceane.fr\/index.php\/%e5%af%a6%e9%a9%97-flow-cytometry-t-cell-phenotypes%ef%bc%88t-cells-subsets%ef%bc%89\/#primaryimage\"},\"thumbnailUrl\":\"https:\/\/myoceane.fr\/wp-content\/uploads\/2020\/05\/T-cell-phenotypes-.png\",\"datePublished\":\"2020-05-09T12:37:16+00:00\",\"dateModified\":\"2020-05-13T08:12:55+00:00\",\"description\":\"To gate in different T cell subsets, you will need to have following CD markers as below (CD Markers panel) with desired fluorochromes. 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